News-Philippines

Regional training course on “Development and validation of analytical method for veterinary drugs and pesticide residues in animal products”, 12–23 November, Philippines

A training course on “Development and validation of analytical method for veterinary drugs and pesticide residues in animal products” was successfully held from 12 to 23 November 2018 at the Bureau of Animal Industry, Quezon City, Philippines.

International Atomic Energy Agency’s RAS5078 project funded this regional training course, and it was hosted by the Government of Philippines through the Bureau of Animal Industry and National Meat Inspection Service of Philippines Department of Agriculture.

Around 30 participants from several member states of RAS 5078 project including Philippines, Bangladesh, China, Indonesia, Jordan, Lao P.D.R., Malaysia, Mongolia, Oman, Pakistan, Sri Lanka, Syrian Arab Republic, Thailand and Vietnam participated in this workshop. All the participants were warmly welcomed at the inauguration ceremony by Dr. Jocelyn A. Salvador, Officer-in-Charge (Assistant Director) of the Bureau of Animal Industry.

Dr. Wim Reybroeck, a senior research scientist at Flanders Research Institute for Agriculture, Fisheries and Food (ILVO) Belgium, and Dwayne Schrunk from Veterinary Diagnostic Laboratory (VDL) of the Iowa State University, USA served as key resource persons in this training program.

The training covered both theoretical and practical aspects of method validation essential for food-safety laboratories. The theory sessions provided the participants with an in-depth knowledge on different protocols used for validating screening and quantitative methods for determining veterinary drugs, European and US regulations pertaining to veterinary drug residues in animal products and trade issues. During the practical sessions the participants received hands on training on sample preparation, extraction of veterinary drugs from different food matrices, application of LC-MS/MS technique for determining veterinary drugs, use of commercially available microbiological and other chemical assays for screening antibiotics in different food matrices, data analysis and interpretation. The theory and practical session were followed by Question and Answer sessions, where resources person and participants shared their experiences.

 A number of screening techniques taught included: Charm MRLBLTET2 – Charm MRL Beta-lactam and Tetracycline 2-MinuteTest (ROSA) and Bioeasy 2IN1 BTCef (EU) Beta-lactams+Tetracyclines Rapid Test for Milk; Premitest on meat juice (meat samples); microbial inhibitor tests (Eclipse Farm 3G with e-reader, Charm Cowside II, Charm Blue Yellow II) and two receptor assays [(CharmMRLBLTET2 and Bioeasy 2IN1 BTCef (EU)); with Eclipse Farm 3G and e-reader and SNAPduo SP Plus; ELISA

Additional work included training on basic parameters for validation of analytical methods (HPLC, GC, GC-MS, and LC-MS techniques) based on US FDA validation guidelines, with some information coming from AOAC validation guidelines. Validation topics included accuracy, precision, recovery, repeatability, reproducibility, stability of standards and compound in matrix. Other parameters for an acceptable calibration curve were discussed along with limit of detection and limit of quantification determination methods.

During the training period, participants also visited the laboratories at the National Meat Inspection Service, and Food Development Center of the National Food Authority with an opportunity to observe facilities and services provided by them.

On the final day of the training, participants described the experience gained through the training, and all of them extended their gratitude to the organizers and the resource persons for the well-organized training program. Participants received certificates from Dr. Carlo A. Arcilla, Director, Philippine Nuclear Research Institute.